46 Year 2: Microbiology exam questions on Untitled Study Note for medical students. Includes MCQs, answers, explanations and written questions. Sample: Which on
This MCQ set contains 46 questions on Untitled Study Note in the Year 2: Microbiology unit. Each question includes the correct answer and a detailed explanation for active recall and exam preparation.
Correct answer: A – Rheumatic fever
Rheumatic fever is a nonsuppurative complication of Group A Streptococcus (Streptococcus pyogenes) pharyngitis. It occurs 2-4 weeks after untreated strep throat due to molecular mimicry, where antibodies against streptococcal M protein cross-react with cardiac tissue, causing carditis, arthritis, and chorea. ---
Correct answer: D – Diphtheria
Corynebacterium diphtheriae produces diphtheria toxin that causes local tissue necrosis in the pharynx, forming a characteristic gray-white pseudomembrane composed of fibrin, dead cells, bacteria, and inflammatory exudate. This membrane adheres firmly to underlying tissue and can cause airway obstruction. ---
Correct answer: E – Rapid-fire cough; pertussis
A paroxysm is a sudden, uncontrollable series of coughs characteristic of pertussis (whooping cough) caused by Bordetella pertussis. The paroxysmal stage features severe coughing fits followed by an inspiratory "whoop" sound, often accompanied by post-tussive vomiting. ---
Correct answer: A – TB exposure
The Mantoux test (tuberculin skin test) detects delayed-type hypersensitivity to tuberculin purified protein derivative (PPD), indicating prior exposure to Mycobacterium tuberculosis. A positive result shows induration at the injection site 48-72 hours after intradermal injection. ---
Correct answer: B – S. pneumoniae
Streptococcus pneumoniae (pneumococcus) is the most common cause of community-acquired bacterial pneumonia. It presents with typical features: acute onset, high fever, productive cough with rusty sputum, pleuritic chest pain, and lobar consolidation on chest X-ray. ---
Correct answer: D – Antitoxin
Botulism requires administration of botulinum antitoxin (trivalent or heptavalent). The antitoxin neutralizes circulating toxin but cannot reverse already bound toxin. Early administration is critical. Antibiotics are ineffective against the preformed toxin. ---
Correct answer: C – Anaerobic
Clostridium perfringens is an obligate anaerobe that multiplies only in oxygen-free environments. Improper food storage (inadequate cooling or reheating) creates anaerobic conditions that allow spore germination and toxin production, leading to food poisoning. ---
Correct answer: D – Cereus
Bacillus cereus causes two forms of food poisoning: the emetic form (1-6 hours, associated with fried rice) caused by cereulide toxin, and the diarrheal form (8-16 hours, associated with meats and vegetables) caused by enterotoxins. ---
Correct answer: D – Inhalation; anthrax
Woolsorter's disease is pulmonary anthrax caused by inhaling Bacillus anthracis spores from contaminated animal products (wool, hides). It causes severe hemorrhagic mediastinitis, septic shock, and high mortality. The name comes from textile workers who processed contaminated wool. ---
Correct answer: D – It inhibits muscle relaxation
Tetanospasmin (tetanus toxin) blocks release of inhibitory neurotransmitters (GABA and glycine) from Renshaw cells in the spinal cord by cleaving synaptobrevin. This prevents muscle relaxation, causing sustained muscle contraction (spastic paralysis) and characteristic lockjaw. ---
Correct answer: B – Gonorrhea; sterility
Salpingitis (fallopian tube inflammation) is commonly caused by Neisseria gonorrhoeae as part of pelvic inflammatory disease (PID). Chronic inflammation causes scarring and tubal obstruction, leading to infertility and increased ectopic pregnancy risk. ---
Correct answer: B – Gonorrhea and chlamydia
Ophthalmia neonatorum (neonatal conjunctivitis) is caused by Neisseria gonorrhoeae and Chlamydia trachomatis acquired during passage through an infected birth canal. Both can cause severe conjunctivitis and, if untreated, corneal scarring and blindness. ---
Correct answer: E – Both B and C are correct
Staphylococcus aureus produces exfoliative toxins causing scalded skin syndrome (bullous impetigo, Ritter's disease) and toxic shock syndrome toxin-1 (TSST-1) causing toxic shock syndrome. Both are toxin-mediated diseases that can result from skin colonization or infection. ---
Correct answer: E – Breathing aerosolized droplets containing the microorganism
The patient has pulmonary tuberculosis caused by Mycobacterium tuberculosis. TB is transmitted by inhalation of aerosolized droplet nuclei (1-5 μm) from an infectious person's cough, sneeze, or speech. The upper lobe cavitation, chronic symptoms, weight loss, and acid-fast bacilli confirm the diagnosis. ---
Correct answer: E – The complex molecular interactions within the biofilm make it difficult to provide effective antimicrobial therapy, and it is likely the catheter will
S. epidermidis biofilms on catheters are highly resistant to antibiotics due to reduced metabolic activity, limited antibiotic penetration through polysaccharide matrix, and altered bacterial physiology. Catheter removal is usually necessary for cure because antibiotics alone cannot eradicate biofilm infections. ---
Correct answer: D – Bacillus anthracis
Robert Koch first proved Bacillus anthracis as the causative agent of anthrax in 1876, establishing Koch's postulates. He isolated the organism from diseased animals, cultured it in pure form, reproduced the disease in healthy animals, and re-isolated the same organism. ---
Correct answer: A – It interacts with macrophages and monocytes yielding release of cytokines
Lipopolysaccharide (LPS/endotoxin) binds to TLR4 on macrophages and monocytes via LPS-binding protein and CD14, triggering massive cytokine release (TNF-α, IL-1, IL-6). This causes fever, shock, and DIC. The toxic component is Lipid A, not the O antigen. ---
Correct answer: A – Lipopolysaccharide is part of the cell wall of Escherichia coli
E. coli is a gram-negative bacterium with LPS in its outer membrane. Cholera toxin is secreted (not on flagella), lecithinase causes tissue necrosis (not diarrhea), and TSST-1 is produced by S. aureus (not S. epidermidis). ---
Correct answer: D – Inhibits elongation factor 2
Diphtheria toxin is an A-B toxin. The A subunit catalyzes ADP-ribosylation of elongation factor 2 (EF-2), irreversibly inactivating it and blocking protein synthesis. One toxin molecule can kill a cell by halting translation. ---
Correct answer: C – Mycobacterium tuberculosis
M. tuberculosis is always pathogenic and never part of normal flora. The others can be normal flora: S. pneumoniae (nasopharynx), E. coli (intestine), S. aureus (skin/nose), N. meningitidis (nasopharynx up to 10% of population). ---
Correct answer: A – Nose
The anterior nares (nose) is the primary reservoir for S. aureus colonization, occurring in 20-40% of healthy adults. Healthcare workers can transmit the organism from their nose to patients via hands, making nasal carriage the most likely source in nosocomial outbreaks. ---
Correct answer: C – Enterococcus faecalis
Enterococcus faecalis is normal intestinal flora and causes urinary tract infections, endocarditis, and intra-abdominal infections, but not diarrhea. C. perfringens, V. cholerae, and E. coli are all well-recognized diarrheal pathogens. ---
Correct answer: D – Ribosome
Ribosomes are internal structures with highly conserved proteins across species, making them poor serological markers. Capsule (K antigens), cell wall (O antigens for LPS), and flagella (H antigens) are surface-exposed and variable, making them excellent for serotyping. ---
Correct answer: C – Pasteurella bettyae
Pasteurella bettyae (formerly CDC group HB-5) is associated with genital tract infections and neonatal infections. P. multocida is primarily associated with animal bites and respiratory infections, not genital tract infections. ---
Correct answer: D – Viridans streptococci are identified by Lancefield grouping, which is based on the carbohydrate in the cell wall
Viridans streptococci lack Lancefield group antigens and cannot be classified by Lancefield grouping. They are identified by biochemical tests, hemolysis pattern, and optochin resistance. Lancefield grouping applies to beta-hemolytic streptococci. ---