Gene Transfer
One of the key ways bacteria acquire new genetic traits, such as antibiotic resistance, is through gene transfer via structural recombination.
Gene Transfer
- Conjugation (plasmids via pili) = most clinically important.
- Transformation = uptake of naked DNA.
- Transduction = bacteriophage transfer.
Read this in Pharmacology Wk 1 & 2
What our notes say
- One of the key ways bacteria acquire new genetic traits, such as antibiotic resistance, is through gene transfer via structural recombination. (Gene Transfer)
- Antimicrobial resistance (AMR) arises through diverse mechanisms like enzymatic inactivation (e.g., beta-lactamases), target modification (e.g., MRSA), and efflux pumps, frequently spread via gene transfer. (Pharmacology Wk 1 & 2)
- Horizontal Gene Transfer (HGT):Conjugation: Bacteria can transfer resistance genes through direct contact, especially via plasmids, between bacterial cells. (Molecular Biology,Molecular Genetics & Microbial-Biochemistry LAQs & SAQs)
- Resistance can arise via mutation or horizontal gene transfer, and involves various biochemical mechanisms. (Principles of Medical Microbiology and Parasitology LAQs & SAQs)
- Sex pili facilitate horizontal gene transfer via conjugation, which may spread antibiotic resistance. (Principles of Medical Microbiology and Parasitology LAQs & SAQs)
- Methods of Gene Transfer:Physical MethodsElectroporation – electrical shock creates pores for DNA entry. (Inhibitors of Translation)
Study material on Gene Transfer
- Gene Transfer
- Types of Mutations in ARBHN (Amino Acid Replacement Based on Hemoglobin to Product Form)
- Gene Transfer and Transcription in Bacteria