Cytopathic Effect
Also written as CPE.
Cytopathic effect (CPE) — cell damage caused by viral replication; used in cell culture diagnosis
What our notes say
- Cytopathic effect (CPE) — cell damage caused by viral replication; used in cell culture diagnosis (EXAM: Bacteriophages, Laboratory Diagnosis, HPV & Parvovirus B19)
- Presence of cytopathic effect is NOT the only way to detect a virus (Medical Virology: Adenoviruses, Hemorrhagic Fever & Viral Receptors)
- Mechanisms of cell damage: direct cytopathic effect (CPE), immune-mediated damage (your own CTLs/inflammation cause the pathology — e.g. (VIROLOGY SEM 3 CRASHED)
- Cell culture: virus grown in susceptible cell lines, identified by characteristic cytopathic effect (e.g. syncytia formation, inclusion bodies, cell rounding) (VIROLOGY SEM 3 CRASHED)
- Cell culture – grow virus in susceptible cell line, observe cytopathic effect (CPE): cell rounding, syncytia (cell fusion, e.g. (Essential Medical Virology: Structure, Classification & Replication)
- Light Microscopy — Cytopathic Effects and Inclusion Bodies: (EXAM: Bacteriophages, Laboratory Diagnosis, HPV & Parvovirus B19)
Study material on Cytopathic Effect
- EXAM: Bacteriophages, Laboratory Diagnosis, HPV & Parvovirus B19
- VIROLOGY SEM 3 CRASHED
- MICROBIOLOGY AND PARASITOLOGY MCQs
Practice questions on Cytopathic Effect
In the absence of cytopathic effects (CPE), how would one demonstrate that a permissive cell is infected by a virus? ---
- By Immunofluorescence Test (IFT)
- By plaque test
- By cell counting
- By hemagglutination assay
- None of the above
Answer: A.